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12-341

Rabbit Anti-Chicken IgY Antibody, HRP conjugate

Upstate®, from rabbit

Synonym(s):

Chicken IgG

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Pack SizeSKUAvailabilityPrice
500 μg
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$268.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
8

$268.00


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biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

chicken

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

dry ice

target post-translational modification

unmodified

General description

Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week′s eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding.

However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.

Application

Detect Chicken IgY using this Rabbit anti-Chicken IgY Antibody, HRP conjugate validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate

Preparation Note

2 years at 4°C

Analysis Note

Routinely evaluated by western blot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
AC146A904612-334
biological source

rabbit

biological source

chicken

biological source

rabbit

biological source

rabbit

species reactivity

chicken

species reactivity

-

species reactivity

chicken

species reactivity

chicken

conjugate

peroxidase conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

unconjugated

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

blocking: suitable (interfering antibodies)

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 (indirect chemiluminescence), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal


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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pelagia Koukouraki et al.
Open biology, 6(4), 160022-160022 (2016-06-02)
Genetic and biochemical studies have established a central role for α-synuclein (SNCA) accumulation in the pathogenesis of Parkinson's disease. Uncovering and subsequently interfering with physiological mechanisms that control SNCA expression is one approach to limit disease progression. To this end
Abhilash I Chiramel et al.
Cell reports, 27(11), 3269-3283 (2019-06-13)
Tripartite motif-containing protein 5α (TRIM5α) is a cellular antiviral restriction factor that prevents early events in retrovirus replication. The activity of TRIM5α is thought to be limited to retroviruses as a result of highly specific interactions with capsid lattices. In contrast
Zhiyong Zhang et al.
Plant biotechnology journal, 18(4), 1056-1065 (2019-10-05)
Gluten-free foods cannot substitute for products made from wheat flour. When wheat products are digested, the remaining peptides can trigger an autoimmune disease in 1% of the North American and European population, called coeliac disease. Because wheat proteins are encoded



Global Trade Item Number

SKUGTIN
12-34104053252589232

Questions

  1. How can we reconstitute this antibody ? 

    1 answer
    1. Reconstitute this product by adding 500µl sterile, distilled water containing 50% glycerol to make a 1mg/ml stock solution. The reconstitution information can be found in the Certificate of Analysis.

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