07-507

Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-Anti-BMK1, Anti-Anti-ERK4, Anti-Anti-ERK5, Anti-Anti-PRKM7
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat, mouse, bovine, human
• Application: WB, inhibition assay
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, bovine, human
• manufacturer/tradename: Upstate^®
• technique(s): inhibition assay: suitable (peptide), western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_139033
• UniProt accession no.: Q13164
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr218/pTyr220)
• Gene Information: bovine ... Mapk7(537703) ; human ... MAPK7(5598) ; mouse ... Mapk7(23939) ; rat ... Mapk7(114509)

Description

General description

110-115kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5

Application

Peptide Inhibition Analysis:
HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.

Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220).

Biochem/physiol Actions

Recognizes phospho-BMK1/Erk5 (Thr218, Tyr220).

Physical form

Affinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at 2-8°C from date of shipment.

Analysis Note

Evaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate.

Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.

Control
Sorbitol-treated HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1