07-405

Anti-PRMT5 Antibody

Upstate^®, from rabbit

• Synonym(s): 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog, SKB1 homolog (S. pombe), Shk1 kinase-binding protein 1 homolog, protein arginine methyltransferase 5, skb1 (S. pombe) homolog
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 85

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_006109
• UniProt accession no.: O14744
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PRMT5(10419)

Description

General description

Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.

PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.

~70 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.

Application

Anti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has been validated in IP & WB.

Immunoprecipitation:
An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

PRMT5, Mr ~70 kDa

Physical form

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Jurkat cell lysate, HeLa cell lysate.

Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Routinely evaluated by Western Blot in RIPA lysates from 3T3/A31, NIH 3T3,HeLa, WI-38, and Jurkat cells.

Western Blot Analysis:
0.5-1 μg/mL of this lot detected PRMT5 in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38, and Jurkat cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABE551

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1