07-328

Anti-acetyl-Histone H4 (Lys8) Antibody

serum, Upstate^®

• Synonym(s): H4K8Ac, Histone H4 (acetyl K8)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, Saccharomyces cerevisiae
• Application: ChIP, DB, IP, WB
• Citations: 92

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, Saccharomyces cerevisiae
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), dot blot: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_175054
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: acetylation (Lys8)
• Gene Information: human ... H4C1(8359)

Description

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

10 kDa

Immunogen

Peptide corresponding to amino acids 3-13 of yeast Histone H4 (RGKGG_AcKGLGKG-C) in which lysine 8 is acetylated.

Application

Use Anti-acetyl-Histone H4 (Lys8) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, IP, WB, DB, ChIP-seq to detect acetyl-Histone H4 (Lys8) also known as H4K8Ac, Histone H4 (acetyl K8).

Analysis Note

Control
Sodium butyrate treated HeLa cells

routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable