Historically, this antibody is provided at a concentration of 1.0mg/mL. Therefore, the 100ug vial will contain 100uL. However, this is subject to change and so the lot-specific certificate of analysis should be consulted for the accurate concentration.
07-2274
Anti-FSP1/S100A4 Antibody
from rabbit
Synonym(s):
S100 calcium binding protein A4, S100 calcium-binding protein A4, fibroblast-specific protein-1, leukemia multidrug resistance associated protein, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), malig
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Check Cart for Availability | $477.00 |
About This Item
eCl@ss:
32160702
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
11
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... S100A4(6275)
mouse ... S100A4(20198)
General description
Fibroblast-specific protein-1 (FSP1)/S100A4 is a cytoplasmic calcium-binding protein belonging to the S100 superfamily of which at least 21 members have been identified so far. Researchers believe that FSP1/S1004A is highly expressed in fibroblasts (Iwano et al., 2001; Strutz et al., 1995), in epithelial and endothelial cells undergoing epithelial-mesenchymal transition (EMT) (Iwano et al., 2002; Zeisberg and Neilson, 2009), and epithelial cancer cells that complete a transition to a metastatic phenotype (Xue et al., 2003).
~ 12 kDa
Immunogen
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to mouse FSP1/S100-A4.
Application
Detect the FSP1/S100A4 protein using this Anti-FSP1/S100A4 validated for use in WB & IHC.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Western Blot Analysis: 1 µg/mL from a previous lot detected S100-A4 on 10 µg of HeLa cell lysate.
Physical form
Format: Purified
Protein G Purified
Purified rabbit polyclonal containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FSP1/S100-A4 on 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FSP1/S100-A4 on 10 µg of NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| clone polyclonal | clone polyclonal | clone 9D5.1, monoclonal | clone polyclonal |
| biological source rabbit | biological source rabbit | biological source mouse | biological source rabbit |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity mouse, human | species reactivity mouse, rat, human, horse | species reactivity human, mouse | species reactivity human |
| Gene Information human ... S100A4(6275) | Gene Information human ... S100A4(6275) | Gene Information human ... S100A4(6275) | Gene Information rat ... S100B(25742) |
| technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Thomas Bärnthaler et al.
The Journal of allergy and clinical immunology, 145(3), 818-833 (2019-12-10)
Idiopathic pulmonary fibrosis (IPF) is a disease with high 5-year mortality and few therapeutic options. Prostaglandin (PG) E2 exhibits antifibrotic properties and is reduced in bronchoalveolar lavage from patients with IPF. 15-Prostaglandin dehydrogenase (15-PGDH) is the key enzyme in PGE2
Tristan Lerbs et al.
JCI insight, 5(16) (2020-08-21)
Scleroderma is a devastating fibrotic autoimmune disease. Current treatments are partly effective in preventing disease progression but do not remove fibrotic tissue. Here, we evaluated whether scleroderma fibroblasts take advantage of the "don't-eat-me-signal" CD47 and whether blocking CD47 enables the
Chuanqi Cai et al.
American journal of physiology. Renal physiology, 318(3), F565-F575 (2019-12-10)
Failure to mature and venous neointimal hyperplasia formation are the two major causes of hemodialysis arteriovenous fistula (AVF) vascular access failure. Percutaneous transluminal angioplasty (PTA) is the firstline treatment for both of these conditions, but, clinically, women have decreased patency
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