07-2268

Anti-phospho-TBC1D1 Antibody (Ser237)

from rabbit, purified by affinity chromatography

• Synonym(s): TBC1 domain family member 1, TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• eCl@ss: 32160702
• UNSPSC Code: 12352203
• NACRES: NA.41
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): ox (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_055988
• UniProt accession no.: Q86TI0
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer237)
• Gene Information: human ... TBC1D1(23216)

Description

General description

TBC1D1 is the first member of a family of proteins that contain the tbc box motif of 180-220 amino acids and may be related to cell growth and differentiation. The composition of TBC1D1 has the same function as a known regulator of insulin-mediated Glut4 translocation and may play a role in severe human obesity. An insulin-independent increase in glucose transport several hours after exercise has been attributed to TBC1D1 phosphorylation. The R125W mutation of TBC1D1 is thought to decrease skeletal muscle glucose transport and could be the main reason for the obesity that seems to be linked to this type of mutation.

~145 kDa observed

Immunogen

Epitope: Phosphorylated Ser237

KLH-conjugated linear peptide corresponding to human TBC1D1 phosphorylated at Ser237.

Application

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Glucose/Glycogen Metabolism

This phospho-TBC1D1 antibody is validated for use in WB for the detection of the phospho-TBC1D1 protein.

Biochem/physiol Actions

This antibody recognizes TBC1D1 phosphorylated at Ser237.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Lambda phosphatase treated and untreated HEK293 cell lysates

Evaluated by Western Blot in lambda phosphatase treated and untreated HEK293 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected TBC1D1 on 10 µg of lambda phosphatase treated and untreated HEK293 cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable