06-520

Anti-Na+/K+ ATPase α-1 Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-CMT2DD
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IP, WB
• Citations: 44

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: bovine, rat, human
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_001001586
• UniProt accession no.: P05023
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: rat ... Atp1A1(24211)

Description

General description

112 kDa

Na+/K+ ATPase α-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation. It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane. It also partners with Src and other signaling proteins. Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades. Binding of ouabain also induces a positive inotropic effect. Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism. (References: Wikipedia and http://www.bioportfolio.com/gene/476-ATP1A1.html)

Immunogen

Epitope: Cytoplasmic domain

GST-tagged ATPase.

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Na+/K+ ATPase α-1 Antibody is validated for use in IC, IP, WB for the detection of Na+/K+ ATPase α-1.

Biochem/physiol Actions

Expected to cross-react with Rat-100%, Mouse-99%; human, pig, rabbit, monkey-96%.

Recognizes the α-1 subunit isoform of Na⁺/K⁺ ATPase.

Physical form

Format: Purified

Protein A purified

Purified antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl and 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Rat Brain Microsomal Prep. lysate.

Routinely evaluated by Immunoprecipitation on rat brain microsomal prep. lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.