06-1041

Anti-RIG-I, CT Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_055129
• UniProt accession no.: O95786
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DDX58(23586)

Description

General description

Retinoic acid-inducible gene 1 (RIG-1, DDX58) is a cytoplasmic protein belonging to the family of RIG-1-like receptor (RLR) family. It is a multidomain protein containing an N-terminal caspase recruitment domain (CARD); a DExD/H box helicase domain; and a C-terminal repression domain. RIG-1 has a well-documented role in innate immunity, as a virus-sensing protein that detects viral RNA molecules and induces the production of IFN-β through activation of the inflammasome complex.

~102 kDa observed

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human RIG-I.

Application

Anti-RIG-I, C-terminus Antibody is an antibody against RIG-I, C-terminus for use in Western Blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

This antibody recognizes the C-terminus of RIG-I.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours.

Evaluated by Western Blot in untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for 12 hours.

Western Blot Analysis: 1 µg/mL of this antibody detected RIG-I in 10 µg of untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours..

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable