05-778

Anti-Rho (-A Antibody, -B, -C), clone 55

clone 55, Upstate^®, from mouse

• Synonym(s): Aplysia ras-related homolog 12, oncogene RHO H12, ras homolog gene family, member A, small GTP binding protein RhoA
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 55, monoclonal
• Application: ICC, WB
• Citations: 52

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 55, monoclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_001655
• UniProt accession no.: P61586
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RHOA(387)

Description

General description

22 kDa

Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.

Immunogen

Recombinant human RhoA containing amino acids 1-155. Clone 55.

Application

Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.

Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Recognizes Rho (-A, -B, -C), Mr 22 kDa.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol.

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
HL-60 cell lysate.

Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1