05-545

Anti-PP2A Antibody, C subunit, clone 7A6

culture supernatant, clone 7A6, Upstate^®

• Synonym(s): Anti-NEDLBA, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7A6, monoclonal
• Application: IP, WB
• Citations: 26

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 7A6, monoclonal
• species reactivity: mouse, Saccharomyces cerevisiae, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_004156
• UniProt accession no.: P67775
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PPP2CA(5515)

Description

General description

36 kDa

Immunogen

peptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit

Application

Anti-PP2A Antibody, C subunit, clone 7A6 detects level of PP2A & has been published & validated for use in IP & WB.

Research Category
Signaling

Neuroscience

Research Sub Category
Kinases & Phosphatases

Neurodegenerative Diseases

Biochem/physiol Actions

Recognizes the catalytic subunit of PP2A.

Physical form

Cell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative.

Unpurified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable