04-300

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate^®, from mouse

• Synonym(s): INSR
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 21g12, monoclonal
• Species reactivity: canine, human, mouse
• Application: ELISA, WB
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 21g12, monoclonal
• species reactivity: canine, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000208.2, NM_001079817.1
• UniProt accession no.: P06213
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr1322)
• Gene Information: human ... INSR(3643)

Description

General description

97 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Application

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Biochem/physiol Actions

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Physical form

Format: Purified

Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.

Subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H₂O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Includes pervanadate treated HEK293 lysate as a positive control.

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids