04-263
Anti-Ubiquitinylated proteins Antibody, clone FK2
clone FK2, Upstate®, from mouse
Synonym(s):
Anti-ubiquitin antibody, FK2 ubiquitin antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FK2, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
General description
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Specificity
Recognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.
Immunogen
Poly-ubiquitinylated-lysozyme.
Application
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins.
Physical form
100 µL of purified immunoglobulin in PBS, pH 7.4 containing 50% glycerol and 0.09% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Format: Purified
Storage and Stability
Stable for 1 year at -20°C from date of shipment for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
Fujimuro, M, et al.
Febs Letters, 349, 173-180 (1994)
Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery.
Kovacs, JR; Li, C; Yang, Q; Li, G; Garcia, IG; Ju, S; Roodman, DG; Windle, JJ; Zhang, X; Lu, B
Cell Death and Differentiation null
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains
Fujimuro, M, et al
European Journal of Biochemistry, 249, 427-433 (1997)
Zhong-Jian Guo et al.
Scientific reports, 5, 14601-14601 (2015-10-07)
Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a
Sungsu Lim et al.
Nature communications, 4, 2641-2641 (2013-10-19)
Gene expression is an intricate process tightly linked from gene activation to the nuclear export of mRNA. Recent studies have indicated that the proteasome is essential for gene expression regulation. The proteasome regulatory particle binds to the SAGA complex and
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