G005902

HPLC Analysis of Sugars by HILIC Chromatography using Ascentis^® Express OH-5 Column with ELSD Detection

application for HPLC

Właściwości

• metody: HPLC: suitable
• parametry testowe: column: Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 μm particles (53778-U); mobile phase: [A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA [B] acetonitrile (30:70, A:B); flow rate: 0.5 mL/min; pressure: 841 psi (58 bar); column temp.: 35 °C; detector: ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( Photomultiplier) Gain 1.0); injection: 10 μL; sample: 0.5 mg/mL each prepared in 50:50 Water:Acetonitrile
• przydatność: application for HPLC
• Zastosowanie: food and beverages

Opis

Komentarz do analizy

This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 column and an Evaporative Light Scattering Detecctor (ELSD) detector.

Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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