Y1751

Yeast Synthetic Drop-out Medium Supplements

without histidine

• Numer MDL: MFCD01095679
• Kod UNSPSC: 12000000
• NACRES: ND.02

Właściwości

• Poziom jakości: 100
• Formularz: powder
• Zastosowanie: food and beverages

Opis

Zastosowanie

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Komponenty

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine. All the amino acids are present at a concentration of 76 mg/lL except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), uracil (76 mg/L), p-aminobenzoic acid (8 mg/L).

Inne uwagi

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Ilość

1.92 g will supplement 1 liter of medium.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)