Products with a high water content away from "the generally known reaction partners of water." This caution is due to water's potential to react violently with numerous substances, resulting in the development of gases and the ignition of released Hydrogen, posing a risk of explosion.
Additionally, various substances or mixtures can explode upon the addition of a drop of water, with details of these reactions provided in the safety data sheets of the specific reaction partners. Substance groups that react violently with water include Alkaline earth metals, Alkaline metals, Aluminum alkalines, Metallic or Nonmetallic hydrides, Metallic or Nonmetallic oxides, Acid anhydrides, and Acid chlorides.
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Kluczowe dokumenty
V5265
Crystal Violet Solution
1%, aqueous solution
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250 ML
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250 ML
371,00 zł
500 ML
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About This Item
Wzór empiryczny (zapis Hilla):
C25H30ClN3
Numer CAS:
Masa cząsteczkowa:
407.98
Beilstein:
3580948
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
NA.47
371,00 zł
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Formularz
aqueous solution
stężenie
1%
metody
microbe id | staining: suitable
kolor
deep violet, purple
Amaks.
0.36-0.44 at 589-594 nm
Zastosowanie
diagnostic assay manufacturing
hematology
histology
temp. przechowywania
room temp
ciąg SMILES
[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
Klucz InChI
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
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Opis ogólny
Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.[1]
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.[2]
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.[2]
Zastosowanie
- Crystal violet is commonly used in Gram staining for the classification of bacteria.[3]
- It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
- It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.[4]
- Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.[1]
- It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.[5][6][7]
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.[8]
Działania biochem./fizjol.
The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.[1]
Zasada
Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
EU REACH Annex XVII (Restriction List)
CAS No.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Characterization of potent and selective iodonium-class inhibitors of NADPH oxidases
Lu J, et al.
Biochemical Pharmacology, 43, 25-38 (2017)
K Engbaek et al.
Journal of clinical pathology, 32(2), 187-190 (1979-02-01)
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be
Histological and histochemical methods: Theory and practice (5th ed.)
JA Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Studies on adsorption of crystal violet dye from aqueous solution onto coniferous pinus bark powder (CPBP)
Ahmad R.
Journal of Hazardous Materials, 171, 767-773 (2009)
Gentian Violet: a 19th century drug re-emerges in the 21st century
Alexander M. Maley, Jack L. Arbiser
Experimental Dermatology, 22(12) (2013)
Powiązane treści
An overview of the science and practice of bacteriology in clinical diagnostics. Learn more about the application of standard and special stains for microscopic analysis.
Przegląd nauki i praktyki bakteriologii w diagnostyce klinicznej. Dowiedz się więcej o zastosowaniu standardowych i specjalnych barwników do analizy mikroskopowej.
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What does "generally known reaction partners of water" refer to in section 10.3 of the V5265 SDS? Additionally, in the SDS for a similar product, C6158, states that violent reactions are possible with strong oxidizing agents, strong acids, and oxidizing agents.
1 answer-
Helpful?
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What solvent is the crystal violet diluted in? Can it be used for staining cells for colony formation assay directly or will fixation with methanol/ethanol be needed prior to the staining?
1 answer-
This product is a 1% solution of Crystal Violet in water. For colony formation assays, it is more common to dissolve the crystal violet in a methanol-based solution. However, it is possible to fix the cells in methanol for 10-15 minutes prior to staining cells with an aqueous Crystal Violet Solution. Some sources recommend substituting absolute ethanol for methanol. Following fixation, the cells can be stained with aqueous Crystal Violet solution.
Helpful?
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What is the shelf life / expiry of this solution?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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