U2383

Ubiquitin Carrier Protein H3/CDC34 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain

• UNSPSC Code: 12352202
• NACRES: NA.56
• MDL number: MFCD03101856
• Form: solution
• Assay: ≥95% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli overproducing strain
• Mol wt: ~32 kDa

Właściwości

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli overproducing strain
• assay: ≥95% (SDS-PAGE)
• form: solution
• mol wt: ~32 kDa
• UniProt accession no.: P49427, P61088
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CDC34(997), UBE2N(7334)

Opis

Biochem/physiol Actions

Cdc34 is an essential gene for viability in yeast. Cdc34 mediates the transition from G₁ to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor, SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunits Cul1 to degrade the NF-κB inhibitor IκBα in a phosphorylation-dependent manner.

Infected cell protein 0 of herpes simplex virus 1 was shown to bind UbcH3 and induce its degradation by promoting its polyubiquination. This resulted in increased expression of an UbcH3-target gene cyclin D1 and aided in further viral infection.

Physical form

Solution in 50 mM HEPES, 200 mM NaCl, 1 mM TCEP (pH 7.5), and 10% glycerol.

Other Notes

Manufactured for Sigma by Boston Biochem. Inc.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable