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T7575

Trypsin Protease

Single-use aliquots ensuring consistent proteolytic activity, suitable for mass spectrometry

Synonim(y):

Proteomics grade, Trypsin, TPCK treated

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Do Państwa/SKUDostępnośćCena netto
1 kit
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1500,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352204
NACRES:
NA.56
Specific activity:
≥10,000 BAEE units/mg protein

1500,00 zł


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Nazwa produktu

Trypsin Singles, Proteomics Grade,

grade

Proteomics Grade

Quality Segment

form

ready-to-use solution

specific activity

≥10,000 BAEE units/mg protein

mol wt

average mol wt 23.29 kDa

optimum pH

~8.0

storage temp.

2-8°C

General description

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment.[1] Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Application

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry).[2] It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.[3]

Features and Benefits

  • Convenient, single-use 1 μg size
  • Eliminates repetitive pipetting
  • Reductively methylated to minimize autolytic activity
  • TPCK treated to quench chymotryptic activity
  • Extensively purified
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EMS0005EMS0004EMS0007
grade

Proteomics Grade

grade

Proteomics Grade

grade

Proteomics Grade

grade

Proteomics Grade

specific activity

≥10,000 BAEE units/mg protein

specific activity

≥10,000 units/mg protein (Enzymatic activity)

specific activity

-

specific activity

≥10,000 units/mg protein

form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

form

lyophilized powder

optimum pH

~8.0

optimum pH

-

optimum pH

-

optimum pH

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

average mol wt 23.29 kDa

mol wt

-

mol wt

-

mol wt

-


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Elementy zestawu są też dostępne oddzielnie

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Opis
Karta charakterystyki & Cennik

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    Karta charakterystyki

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

3 - Flammable liquids

wgk

WGK 2



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Questions

  1. How many 1 µg samples are included in the proteomics grade Trypsin kit (T7575-1KT)?

    1 answer
    1. The proteomics grade Trypsin kit (T7575-1KT) includes 96 individual 1 µg samples, each provided in a single vial.

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