This product is soluble in water at approximately 2 mg/ml.
T5771
Testosterone 3-(O-carboxymethyl)oxime: BSA - fluorescein isothiocyanate conjugate
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Pozwól nam pomócassay
≥98.00% (TLC)
form
powder
drug control
regulated under CDSA - not available from Sigma-Aldrich Canada
extent of labeling
~3 mol FITC per mol BSA, ~10 mol steroid per mol BSA
technique(s)
flow cytometry: suitable
solubility
water: 1.90-2.10 mg/mL, clear to faintly hazy, orange
shipped in
ambient
storage temp.
2-8°C
Quality Level
1 of 4
Ta pozycja | |||
|---|---|---|---|
| assay ≥98.00% (TLC) | assay ≥98.00% (TLC) | assay ≥98% (TLC) | assay ≥98.00% (TLC) |
| form powder | form powder | form lyophilized powder | form powder |
| drug control regulated under CDSA - not available from Sigma-Aldrich Canada | drug control - | drug control regulated under CDSA - not available from Sigma-Aldrich Canada | drug control regulated under CDSA - not available from Sigma-Aldrich Canada |
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. room temp |
Application
Testosterone 3-(O-carboxymethyl)oxime: BSA-FITC has been used for flow cytometric assays in serum-free cell cultures2. It has also been used for fluorescence labeling experiments in L6 cells3.
Biochem/physiol Actions
Studies have reported that BSA-coupled testosterone can block the growth of LNCaP cells. Furthermore, it can activate Fas protein and cell death, as well as reduce the translocation, adhesion and incursion of iAR-negative, DU145 prostate cancer cells2.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Rui Fu et al.
Journal of cellular physiology, 227(1), 98-107 (2011-03-05)
Androgens are known to modulate the skeletal muscle proliferation and differentiation processes. Recent in vitro studies have shown that dihydrotestosterone and anabolic steroids have functions in promoting the proliferation and differentiation of the mouse skeletal muscle myoblast C2C12 cell line
Anastassia Hatzoglou et al.
The Journal of clinical endocrinology and metabolism, 90(2), 893-903 (2004-12-09)
Nongenomic androgen actions imply mechanisms different from the classical intracellular androgen receptor (iAR) activation. We have recently reported the identification of a membrane androgen receptor (mAR) on LNCaP human prostate cancer cells, mediating testosterone signal transduction within minutes. In the
Yizhou Zhang et al.
Aging, 11(8), 2281-2294 (2019-04-22)
The non-genomic actions of androgen-induced synaptic plasticity have been extensively studied. However, the underlying mechanisms remain controversial. We recently found that testosterone-fetal bovine serum albumin (T-BSA), a cell membrane-impermeable complex, led to a rapid increase in the postsynaptic density 95
Synthesis of fluorescein labelled steroid hormone-albumin conjugates for the fluorescent histochemical detection of hormone receptors.
E Gaetjens et al.
Journal of steroid biochemistry, 13(8), 1001-1003 (1980-08-01)
Sha Li et al.
Molecular and cellular endocrinology, 414, 82-90 (2015-07-15)
Testosterone (T), the principal androgen, and its metabolite, dihydrotestosterone (DHT), are known to mediate their effects through binding to intracellular androgen receptors (iARs). In addition to their well-known genomic effects, androgens rapidly alter neuronal excitability through a non-genomic pathway mediated
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How will you dissolve this product (T5771)?
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How can I dissolve the products that arrived in solid form?
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The spec sheet indicates that the product is tested for solubility at 2mg/ml in water. For Testosterone 3-(O-carboxymethyl)oxime: BSA, solubility is tested in Phosphate buffer pH 7.5. Additionally, without the albumin conjugate, Testosterone 3-(O-carboxymethyl)oxime is soluble in ethanol at 50mg/ml.
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