SRP3238

EGF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonim(y): Epidermal Growth Factor, URG, Urogastrone
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: rat
• rekombinowane: expressed in E. coli
• Próba: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Postać: lyophilized
• siła działania: ≤0.1 ng/mL
• masa cząsteczkowa: 6.2 kDa
• opakowanie: pkg of 100 μg
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: <0.1 EU/μg endotoxin, tested
• kolor: white to off-white
• numer dostępu UniProt: P07522
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• informacje o genach: rat ... EGF(25313)

Opis

Opis ogólny

Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide. Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.

Zastosowanie

Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.

Działania biochem./fizjol.

Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).

Sekwencja

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Postać fizyczna

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Rekonstytucja

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable