Ludzka kinaza sfingozyny 1 (GenBank Accession No. AF200328), pełnej długości z N-końcowym znacznikiem His, MW = 46,9 kDa, wyrażona w systemie ekspresji komórek Sf9 zakażonych bakulowirusem.
Zastosowanie
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Definicja jednostki
Jedna jednostka aktywności kinazy sfingozynowej jest definiowana jako ilość enzymu wymagana do wytworzenia 1 pmol S1P/min.
Postać fizyczna
Formuła w 25 mM Tris-HCl, pH 8,0, 100 mM NaCl, 0,05% Tween-20, 50% glicerolu i 3 mM DTT.
Uwaga dotycząca przygotowania
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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