This product is not specifically tested for enzyme inhibition but is expected to be suitable in enzyme inhibition screening. Please see the link below to review several publications that may be helpful:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=srp0122+sigma&btnG=
Przejdź do
SRP0122
LSD1 Active human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonim(y):
Amine oxidase (flavin containing) domain 2 (AOF2), BRAF35-HDAC complex protein BHC110, KIAA0601, Lysine (K)-specific demethylase 1 (LSD1), Lysine-specific histone demethylase 1A (KDM1A)
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
50 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2770,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352202
2770,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
103 kDa
packaging
pkg of 50 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... KDM1A(23028)
General description
Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥70% (SDS-PAGE) | description from rabbit, purified by affinity chromatography | description recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE) | description recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE) |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells |
| assay ≥70% (SDS-PAGE) | assay - | assay ≥40% (SDS-PAGE) | assay ≥40% (SDS-PAGE) |
| biological source human | biological source rabbit | biological source human | biological source human |
| form aqueous solution | form - | form aqueous solution | form aqueous solution |
| storage temp. −70°C | storage temp. - | storage temp. −70°C | storage temp. −70°C |
| mol wt 103 kDa | mol wt - | mol wt 148.2 kDa | mol wt 155 kDa |
Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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This enzyme can be used for enzyme inhibition assays? If yes, is there any cited paper your product?
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Helpful?
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