SAE0152

Lysozyme from chicken egg white

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥40,000 units/mg protein

• Synonim(y): Lysozyme from chicken egg white, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• Numer CAS: 12650-88-3
• Numer EC enzymu: 3.2.1.17 (BRENDA, IUBMB)
• Numer MDL: MFCD00131557
• Kod UNSPSC: 12352204
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: chicken egg white
• Poziom jakości: 100
• Formularz: powder
• aktywność właściwa: ≥40000 units/mg protein
• masa cząsteczkowa: single-chain 14.3 kDa
• Właściwości: DNA free
• metody: cell based assay: suitable
• przydatność: suitable for cell lysis
• Zastosowanie: cell analysis
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).

Lysozyme activity: ≥40,000 units/mg protein.

For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Zastosowanie

• Lysozyme is used for the extraction of genomic DNA from bacterial cells.
• Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
• Lysonzyme is used to prepare spheroplasts.

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Działania biochem./fizjol.

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Cechy i korzyści

Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.

Definicja jednostki

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable