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Merck

SAB4200766

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal

clone NOS-IN, purified from hybridoma cell culture

Synonim(y):

Anti-Hepatocyte NOS (HEP-NOS), Anti-Inducible NOS (iNOS), Anti-NOS type II, Anti-Peptidyl-cysteine S-nitrosylase NOS2

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified from hybridoma cell culture

rodzaj przeciwciała

primary antibodies

klon

NOS-IN, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

~130 kDa

reaktywność gatunkowa

human, mouse, rat, plant

stężenie

~1.0 mg/mL

metody

flow cytometry: suitable
immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections

izotyp

IgG1

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

mouse ... Nos2(18126)

Opis ogólny

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain.

Specyficzność

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin.

Immunogen

synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH

Zastosowanie

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • fluorescence-activated cell sorting (FACS)

Działania biochem./fizjol.

Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO) via an OH-L-arginine intermediate in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen. Elevated levels of iNOS is observed in asthma. A high level of iNOS leads to an increase in NO production which may contribute to cell death and tissue damage. It is also implicated in the pathophysiology of septic shock. Single nucleotide polymorphism in the iNOS gene is indicative of high risk for B and T-cell non-Hodgkin lymphoma. iNOS also elicits macrophage bactericidal functionality.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

iNOS (NOS2) at a glance
Lowenstein CJ and Padalko E
Journal of Cell Science, 117(14), 2865-2867 (2004)
Christian Bogdan
Trends in immunology, 36(3), 161-178 (2015-02-18)
Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO
Cleva Villanueva et al.
Free radical biology & medicine, 49(3), 307-316 (2010-04-15)
The effects of nitric oxide in biological systems depend on its steady-state concentration and where it is being produced. The organ where nitric oxide is produced is relevant, and within the organ, which types of cells are actually contributing to
Federica Vannini et al.
Redox biology, 6, 334-343 (2015-09-04)
Nitric oxide (NO) is one of the 10 smallest molecules found in nature. It is a simple gaseous free radical whose predominant functions is that of a messenger through cGMP. In mammals, NO is synthesized by the enzyme nitric oxide
Jyotsna Batra et al.
Indian journal of biochemistry & biophysics, 44(5), 303-309 (2008-03-18)
Asthma is one of the most common chronic inflammatory disorder of the airways of the lungs, affecting more than 300 million people all over the world. Nitric oxide (NO) is endogenously produced in mammalian airways by nitric oxide synthase (NOS)

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