SAB4200283

Anti-Tensin 1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonim(y): Anti-TNS1
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen 225 kDa (rat); antigen ~150 kDa (human)
• reaktywność gatunkowa: rat, human, chicken
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• stężenie: ~1.0 mg/mL
• metody: immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart.; indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells.; western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1
• numer dostępu UniProt: Q9HBL0
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TNS1(7145) ; mouse ... Tns1(21961) ; rat ... Tns1(301509)

Opis

Opis ogólny

Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs).

Immunogen

synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.

Zastosowanie

Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry.

Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting.

Działania biochem./fizjol.

Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways.

Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable