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RAB0210

Mouse Granzyme B ELISA Kit

for serum, plasma and cell culture supernatant

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1 KIT

2890,00 zł

2890,00 zł


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Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Gzmb(14939)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice

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Pomoc techniczna
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Pozwól nam pomóc

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Gzmb(14939)

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1 of 4

Ta pozycja
RAB0104RAB0224RAB0125
species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

Gene Information

mouse ... Gzmb(14939)

Gene Information

mouse ... Csf3(12985)

Gene Information

mouse ... Ifng(15978)

Gene Information

mouse ... Cxcl12(20315)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/mL
standard curve range: 14.34-3500 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.62-150 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 9.38-600 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Mouse Granzyme B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Granzyme B in serum, plasma, cell culture supernatants and urine.
The Mouse Granzyme B enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of mouse granzyme B in serum, plasma, cell culture supernatants and urine. Granzyme B binds to mannose-6-phosphate/insulin-like growth factor II receptor. This protein is implicated in fast DNA fragmentation, a key step towards apoptosis in cells. Granzyme B is involved in the cleaving of pro-caspase-3, which leads to the activation of caspase-3. This protein also cleaves caspase-6, -7, -8, -9, and -10.[1]

Immunogen

Recombinant Mouse Granzyme B

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)Karta charakterystyki

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)Karta charakterystyki

  • RABSTOP3ELISA Stop Solution (Item I)Karta charakterystyki

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)Karta charakterystyki

  • RABWASH420X Wash Buffer (Item B)Karta charakterystyki

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Klasa składowania

10 - Combustible liquids


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Kosei Hasegawa et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 41(4), 201-207 (2018-02-13)
Cancer immunotherapy has now been established as a leading standard therapeutic option in a subset of patients with cancer. In this study, we conducted a phase I dose-escalation trial using a mixture of 5 peptides to vaccinate cervical cancer patients
Hannah Jonescheit et al.
Cells, 9(5) (2020-05-10)
Pancreatic ductal adenocarcinoma (PDAC) is a malignant gastrointestinal disease. The enzyme indoleamine-2,3-dioxgenase (IDO) is often overexpressed in PDAC and its downstream metabolite kynurenine has been reported to inhibit T cell activation and proliferation. Since γδ T cells are of high
Patrick R Hagner et al.
British journal of haematology, 179(3), 399-409 (2017-08-05)
Lenalidomide is an immunomodulatory agent that has demonstrated clinical benefit for patients with relapsed or refractory mantle cell lymphoma (MCL); however, despite this observed clinical activity, the mechanism of action (MOA) of lenalidomide has not been characterized in this setting.
Sarah J Lord et al.
Immunological reviews, 193, 31-38 (2003-05-20)
A main pathway used by cytotoxic T lymphocytes (CTLs) and natural killer cells to eliminate pathogenic cells is via exocytosis of granule components in the direction of the target cell, delivering a lethal hit of cytolytic molecules. Amongst these, granzyme
Ciputra Adijaya Hartana et al.
PloS one, 13(7), e0200079-e0200079 (2018-07-03)
The immune system plays a significant role in urothelial bladder cancer (UBC) progression, with CD8+ T cells being capable to directly kill tumor cells using perforin and granzymes. However, tumors avoid immune recognition by escape mechanisms. In this study, we

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Questions

  1. It says here that this ELISA kit works for the detection of GzmB in mouse serum, but I can't find any papers that have used serum. Could you please cite a paper or previous work demonstrating the utility of this kit for detecting GzmB in serum?

    1 answer
    1. Unfortunately, published citations on the use of this kit with mouse serum are not available. Please see the image below for an example of expected recovery rates:

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