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Merck

R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Synonim(y):

Transfer RNA, tRNA

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352106
NACRES:
NA.55

klasa czystości

for molecular biology

Poziom jakości

typ

Type V

Postać

lyophilized powder

aktywność właściwa

15-19 units/mg solid

warunki przechowywania

desiccated

rozpuszczalność

H2O: ~2 mg/mL, clear to hazy

temp. przechowywania

2-8°C

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Opis ogólny

Wheat germ tRNA contains many different specific tRNA molecules.

Zastosowanie

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Komponenty

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Definicja jednostki

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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