R4752
Ribonucleic acid, transfer from bovine liver
Type XI, lyophilized powder
Synonim(y):
Transfer RNA, tRNA
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Informacje o tej pozycji
Form:
lyophilized powder
Storage temp.:
−20°C
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Type XI
Quality Level
form
lyophilized powder
color
white to light yellow
suitability
suitable for (gives a positive response when incubated at pH 7.6 at 37°C with aminoacyl-tRNA synthetase and the amino acid L-arginine)
storage temp.
−20°C
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General description
Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.
Application
Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
Ribonucleic acid, transfer from bovine liver has been used:
- to analyze the efficiency of thioflavin T (ThT) as a fluorophore
- to test its ability to inhibit the growth of endothelial cells
- as a component in translation mixture
- as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column
Biochem/physiol Actions
Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.
Analysis Note
This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine
Other Notes
One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
New role for tRNA and its fragment purified from human urinary bladder carcinoma conditioned medium: inhibition of endothelial cell growth
Zhao H, et al.
Journal of Cellular Biochemistry, 76(1), 109-117 (2000)
Approaches for monitoring nuclear translation
mRNA Processing and Metabolism, 103-113 (2004)
From DNA to Protein: The Transfer of Genetic Information, 217-221 (1980)
RNA-Protein Interactions : A Practical Approach, 149-150 (1998)
Molecular Biology of the Transfer RNA Revisited, 5-7 (2014)
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