PURE1A
Protein A Antibody Purification Kit
sufficient for 10 purifications
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Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352203
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Pozwól nam pomócusage
sufficient for 10 purifications
shipped in
wet ice
storage temp.
2-8°C
General description
The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.
Application
PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.
Biochem/physiol Actions
Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.
Features and Benefits
- High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
- Specific - will only bind IgG
- Easy to use - antibody is eluted and desalted in a single step, ready to use
- Gentle - avoids prolonged exposure of the antibody to low pH
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
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Tylko elementy zestawu
Numer produktu
Opis
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
Zobacz wszystko (8)
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 1
Klasa składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
target_organs
Eyes,Central nervous system
wgk
WGK 3
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Protokoły
Protokoły oczyszczania przeciwciał dają preparaty zawierające endogenne IgG wraz ze specyficznymi przeciwciałami.
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
Powiązane treści
Instructions
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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