The PKH cell linker kits have not been specifically tested for the detection of circulatory fibrocytes. However, there are several publications that indicate suitability. Please see the link below to review one such reference:
https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13080
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PKH26PCL
PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling
Distributed for Phanos Technologies
Synonim(y):
Etykieta komórek fagocytarnych
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About This Item
Kod UNSPSC:
12352207
NACRES:
NA.32
5110,00 zł
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Zastosowanie
This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.
Zasada
The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.
Powiązanie
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Informacje prawne
CellVue is a registered trademark of Phanos Technologies
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Tylko elementy zestawu
Numer produktu
Opis
- Diluent B 6 x 10
- PKH26 cell linker in ethanol .5 mL
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Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Flam. Liq. 2
Kod klasy składowania
3 - Flammable liquids
Temperatura zapłonu (°F)
57.2 °F - closed cup
Temperatura zapłonu (°C)
14.0 °C - closed cup
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Produkty
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
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Is this kit suitable for label circulatory fibrocyte isolated from rat PBMC?
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