PCRISPR006
Human CRISPR Poison Exon Knockout Library
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About This Item
Kod UNSPSC:
41105904
NACRES:
NE.02
Polecane produkty
opakowanie
pkg of 5 vials (5x200µL aliquots )
stężenie
≥5x108 VP/ml (via p24 Assay)
Zastosowanie
CRISPR
Warunki transportu
dry ice
temp. przechowywania
−70°C
Opis ogólny
The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions. This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Zastosowanie
- Functional Genomics/Target Validation
- Dual-gRNA
- Poison exon
- Internal controls included
Cechy i korzyści
- Focus on your research, and we will generate your lentivirus screening library
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Built-in enrichment and depletion controls allow researchers to gauge the success of their pooled screening experiments confidently.
- Dual-Guide Vector System (pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO.
- Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.
Zasada
In traditional CRISPR KO screens, Cas9 introduces double-strand breaks at locations specified by a gRNA. In this library, a CRISPR-Cas9-based method is used by expressing two sgRNAs to manipulate isoforms independent of gene inactivation. This approach enables rapid suppression of exon recognition in polyclonal settings to identify functional roles for individual exons.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
James D Thomas et al.
Nature genetics, 52(1), 84-94 (2020-01-09)
While RNA-seq has enabled comprehensive quantification of alternative splicing, no correspondingly high-throughput assay exists for functionally interrogating individual isoforms. We describe pgFARM (paired guide RNAs for alternative exon removal), a CRISPR-Cas9-based method to manipulate isoforms independent of gene inactivation. This
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