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P0072

Sigma-Aldrich

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~51 kDa

reaktywność gatunkowa

rat (predicted), mouse (predicted), human

stężenie

~1 mg/mL

metody

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS
western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS

numer dostępu UniProt

Q53EL6

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

phosphorylation (pSer67)

informacje o genach

human ... PDCD4(27250) , SELP(6403)
mouse ... Pdcd4(18569)

Opis ogólny

Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.

Zastosowanie

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.

Działania biochem./fizjol.

Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21Waf1/Cip1
Goke R, et al.
American Journal of Physiology. Cell Physiology, C1541-C1546 (2004)
S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
Dorrello NV, et al.
Science (New York, N.Y.), 314(5798), 467-471 (2006)
Programmed Cell Death 4 (PDCD4): A Novel Player in Ethanol Mediated Suppression of Protein Translation in Primary Cortical Neurons and Developing Cerebral Cortex
Narasimhan M, et al.
Alcoholism, Clinical and Experimental Research, 37(1), 96-96 (2013)
Cory M Howard et al.
Frontiers in oncology, 9, 284-284 (2019-05-21)
Triple-negative breast cancer (TNBC) remains clinically challenging as effective targeted therapies are lacking. In addition, patient mortality mainly results from the metastasized lesions. CXCR4 has been identified to be one of the major chemokine receptors involved in breast cancer metastasis.
Jesse J Chen et al.
The Journal of biological chemistry, 286(47), 40867-40877 (2011-10-05)
Ubiquitin-activating enzyme (UAE or E1) activates ubiquitin via an adenylate intermediate and catalyzes its transfer to a ubiquitin-conjugating enzyme (E2). MLN4924 is an adenosine sulfamate analogue that was identified as a selective, mechanism-based inhibitor of NEDD8-activating enzyme (NAE), another E1

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