N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• Synonim(y): Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• Numer MDL: MFCD07370972
• Kod UNSPSC: 12352200
• NACRES: NA.32

Właściwości

• rekombinowane: expressed in E. coli
• Poziom jakości: 100
• Próba: ≥80% (SDS-PAGE)
• Postać: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• masa cząsteczkowa: protein apparent mol wt ~58 kDa
• numer dostępu UniProt: Q9HC98
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... NEK6(10783)

Opis

Zastosowanie

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Działania biochem./fizjol.

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

Definicja jednostki

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Postać fizyczna

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable