N4645

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

• Synonim(y): p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
• Wzór liniowy: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Numer CAS: 333338-18-4
• Masa cząsteczkowa: 371.14
• UNSPSC Code: 12352204
• NACRES: NA.75
• PubChem Substance ID: 24897708
• EC Number: 224-246-5
• Beilstein/REAXYS Number: 5324661
• MDL number: MFCD00066288

Właściwości

• biological source: synthetic (organic)
• Quality Segment: 200
• product line: BioReagent
• assay: ≥97%
• form: powder
• technique(s): cell culture | mammalian: suitable
• solubility: H₂O: 100 mg/mL
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Opis

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)