MAK299

NNMT Inhibitor Screening Assay Kit

sufficient for 100 fluorometric tests

• NACRES: NA.84
• UNSPSC Code: 12352200

Właściwości

• detection method: fluorometric
• relevant disease(s): endocrinological disorders, diabetes; obesity; cancer; neurological disorders
• storage temp.: −70°C

Opis

General description

N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.

Application

Suitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).

Biochem/physiol Actions

This NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.

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Informacja o środkach bezpieczeństwa

• signalword: Danger
• Klasa składowania: 3 - Flammable liquids
• flash_point_f: 84.2 °F
• flash_point_c: 29 °C
• wgk: WGK 3
• pictograms: GHS02,GHS08,GHS05,GHS07,GHS09
• hcodes: H226,H312,H317,H318,H334,H361f,H411,EUH430
• pcodes: P201 - P202 - P210 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Dermal - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1