Przejdź do zawartości
Merck

Kluczowe dokumenty

Wyświetl całą dokumentację

Przejdź do

MAK169

High Sensitivity Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests

Synonim(y):

Pyrophosphate (PPi) Assay Kit

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok
Do Państwa/SKUDostępnośćCena netto
1 kit
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2700,00 zł

Informacje o tej pozycji

UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84

2700,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
​
Złóż zamówienie na produkt na zamówienie
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc

usage

sufficient for 200 fluorometric tests

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

genitourinary disorders; rheumatological diseases

storage temp.

−20°C

General description

Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Application

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.

Biochem/physiol Actions

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja
MAK168MAK135
High Sensitivity Pyrophosphate Assay Kit sufficient for 200 fluorometric tests

Sigma-Aldrich

MAK169

High Sensitivity Pyrophosphate Assay Kit

Pyrophosphate Assay Kit sufficient for 200 fluorometric tests (Blue fluorescence)

Sigma-Aldrich

MAK168

Pyrophosphate Assay Kit

ADP/ATP Ratio Assay Kit sufficient for 100 tests (bioluminescent)

Sigma-Aldrich

MAK135

ADP/ATP Ratio Assay Kit

description

sufficient for 200 fluorometric tests

description

sufficient for 200 fluorometric tests (Blue fluorescence)

description

sufficient for 100 tests (bioluminescent)

usage

sufficient for 200 fluorometric tests

usage

sufficient for 200 fluorometric tests (Blue fluorescence)

usage

sufficient for 100 tests (bioluminescent)

detection method

fluorometric

detection method

fluorometric

detection method

chemiluminescent

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

pharmaceutical

relevant disease(s)

genitourinary disorders; rheumatological diseases

relevant disease(s)

-

relevant disease(s)

cancer

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Questions

1–2 of 2 Questions  

  1. Can this kit be used to quantify pyrophosphate in cell extracts? If so, are there any recommendations for sample preparation?

    1 answer

    1. The kit has not been validated for use with cell lysates. No supporting data are currently available for this sample type. The end user would have to determine suitability. Please refer to the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/749/mak169bul.pdf

      Please see the link below to review product C3228, CelLytic Cell Lysis Reagent for use with Mammalian cell/tissues:
      https://www.sigmaaldrich.com/US/en/product/sigma/c3228

      Please see the following link to review the C3228 cell lysis protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Helpful?

  2. The product info sheet recommends 10-30min incubation after probe addition. Is this kit therefore amenable to continuous read formats or is signal development slow? Also, is there a recommended quenching method for this kit?

    1 answer

    1. This kit functions using an endpoint determination. Fluorescence intensity should be measured a single time between 10 and 30 minutes after the addition of the master reaction mix. The reaction signal remains stable for 2 hours. No recommended quenching method is available.

      Helpful?

Reviews

No rating value

Active Filters