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MAK168

Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests (Blue fluorescence)

Synonim(y):

High-Sensitivity Pyrophosphate Assay Kit

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Informacje o tej pozycji

UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84
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usage

sufficient for 200 fluorometric tests (Blue fluorescence)

application(s)

cosmetics
food and beverages

detection method

fluorometric

storage temp.

−20°C

General description

Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.

Application

The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.

Biochem/physiol Actions

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
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MAK169MAK126MAK118
description

sufficient for 200 fluorometric tests (Blue fluorescence)

description

sufficient for 200 fluorometric tests

description

sufficient for 180 colorimetric tests

description

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 200 fluorometric tests (Blue fluorescence)

usage

sufficient for 200 fluorometric tests

usage

sufficient for 180 colorimetric tests

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

fluorometric

detection method

fluorometric

detection method

colorimetric

detection method

colorimetric, fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

cosmetics
environmental
food and beverages


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

188.6 °F

flash_point_c

87 °C



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Any challenges encountered with decreases in fluorescence due to buffer components in the assays, such as manganese (1 mM), DTT (5 mM), and the combination of manganese with a reducing agent (both BME and DTT)? It seems that the signal is significantly reduced, if not entirely abolished, when these components are included in the buffer, even with substantial amounts of the PPi standard.

    1 answer
    1. Considering the significant quenching of fluorescence by manganese (Mn), it is not suitable for the assay. Instead, the use of MgCl2 (up to 1 mM) is suggested as it offers less interference. Additionally, DTT can be used up to 1 mM.

      Helpful?

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