MAK161

Multidrug Resistance Assay Kit

(Fluorometric MDR Assay)

• Synonim(y): MDR Assay
• Kod UNSPSC: 12161503
• NACRES: NA.84

Właściwości

• zastosowanie: (Fluorometric MDR Assay)
• Zastosowanie: pharmaceutical
• metoda wykrywania: fluorometric
• powiązane choroby: cancer
• temp. przechowywania: −20°C
• informacje o genach: human ... ABCB1(5243) , ABCC2(1244) ; mouse ... ABCC2(12780) ; rat ... abcc2(25303)

Opis

Opis ogólny

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex = 490/λ_em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Cechy i korzyści

Compatible with high-throughput handling systems.

Przydatność

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Zasada

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex = 490/λ_Em = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids