If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
L9150
N-Lauroylsarcosine sodium salt
detergent for use in cell lysis
Synonim(y):
Sodium lauroyl sarcosinate, N-Dodecanoyl-N-methylglycine sodium salt, Sarkosyl NL
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InChI key
KSAVQLQVUXSOCR-UHFFFAOYSA-M
InChI
1S/C15H29NO3.Na/c1-3-4-5-6-7-8-9-10-11-12-14(17)16(2)13-15(18)19;/h3-13H2,1-2H3,(H,18,19);/q;+1/p-1
SMILES string
[Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O
grade
Molecular Biology
description
anionic
product line
BioReagent
assay
≥94%
form
powder
mol wt
micellar avg mol wt 600
aggregation number
2
technique(s)
protein purification: suitable, protein quantification: suitable
CMC
14.6 mM (20-25°C)
solubility
H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
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Ta pozycja | |||
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| assay ≥94% | assay ≥99.0% (HPLC) | assay ≥97% (TLC) | assay ≥97.0% (HPLC) |
| technique(s) protein purification: suitable, protein quantification: suitable | technique(s) - | technique(s) - | technique(s) protein expression: suitable, protein purification: suitable, protein quantification: suitable |
| form powder | form powder | form powder | form powder |
| aggregation number 2 | aggregation number 2 | aggregation number 2 | aggregation number 2 |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. - |
General description
This amino acid-type anionic surfactant, also recognized as a fatty acid amide and detergent, exhibits protein denaturant potency and has been integrated into lysing solutions, such as the cornet assay. Its application extends to lysing protoplasts for chromosomal DNA isolation from Streptococcus mutans. Notably, Lauroylsarcosine Sodium Salt, characterized by its ionic detergent properties, is commonly employed for purifying membrane proteins. Additionally, Sodium Lauroyl Sarcosine also plays a crucial role in concentrated salt solutions, facilitating cell lysis in RNA purification protocols and membrane solubilization. Whether it′s breaking down cell structures or facilitating membrane protein purification, this mild surfactant proves valuable in various applications within the realms of biology and biochemistry.
Application
- solubilization and separation of membrane proteins
- lysis of cells during the isolation of RNA
- inhibition of hexokinase
Features and Benefits
- Suitable for Molecular Biology, Biochemical and Cell Biology research
- Can be used for Protein Purification and Quantification
- High purity product for research applications
Other Notes
comparable product
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Inhalation - Eye Dam. 1 - Skin Irrit. 2
Klasa składowania
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 1
flash_point_f
512.6 °F - closed cup
flash_point_c
267 °C - closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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