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L9017

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

Synonim(y):

Phaseolus vulgaris agglutinin, PHA

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1 MG
311,00 zł
5 MG
986,00 zł
10 MG
1810,00 zł

311,00 zł

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Zmień widok
1 MG
311,00 zł
5 MG
986,00 zł
10 MG
1810,00 zł

About This Item

Numer WE:
232-718-7
Kod UNSPSC:
12352202
NACRES:
NA.32

311,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

pochodzenie biologiczne

Phaseolus vulgaris

linia produktu

BioXtra

Formularz

lyophilized powder

siła działania

≤17 μg per mL agglutination activity

oczyszczone przez

affinity chromatography

zanieczyszczenia

salt, essentially free

temp. przechowywania

2-8°C

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Opis ogólny

Lectins are carbohydrate-binding proteins,[1] that contains four subunits with a molecular weight of 125 kDa.[2] Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.[3]

Zastosowanie

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for phytohemagglutinin (PHA) swelling assay[4]
  • for lymphocyte activation assay[5]
  • to evaluate cell-mediated immune response[6]

Działania biochem./fizjol.

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.[1] It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.[7]
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Jakość

Mitogenic activity at ≤10 μg per mL.

Komentarz do analizy

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000441036
0000441034
0000441034
0000441036
0000407585

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Diane L Bolton et al.
PLoS pathogens, 13(6), e1006445-e1006445 (2017-06-28)
CD4 T cells harboring HIV-1/SIV represent a formidable hurdle to eradicating infection, and yet their detailed phenotype remains unknown. Here we integrate two single-cell technologies, flow cytometry and highly multiplexed quantitative RT-PCR, to characterize SIV-infected CD4 T cells directly ex
Jon S Beadell et al.
PloS one, 2(9), e896-e896 (2007-09-20)
Dramatic declines of native Hawaiian avifauna due to the human-mediated emergence of avian malaria and pox prompted an examination of whether island taxa share a common altered immunological signature, potentially driven by reduced genetic diversity and reduced exposure to parasites.
Chickadees faced with unpredictable food increase fat reserves but certain components of their immune function decline
Cornelius EA, et al.
Physiological and Biochemical Zoology : Pbz, 90(2), 190-200 (2017)
The Lectins: Properties, Functions, and Applications in Biology and Medicine (2012)
Charlotte D C C van der Heijden et al.
The Journal of clinical endocrinology and metabolism, 105(5) (2019-12-26)
Primary aldosteronism (PA) confers an increased risk of cardiovascular disease (CVD), independent of blood pressure. Animal models have shown that aldosterone accelerates atherosclerosis through proinflammatory changes in innate immune cells; human data are scarce. The objective of this article is
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Produkty

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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