I5513

Imidazole

for molecular biology, ≥99% (titration)

• Synonim(y): 3-Azapyrrole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
• Wzór empiryczny (zapis Hilla): C₃H₄N₂
• Numer CAS: 288-32-4
• Masa cząsteczkowa: 68.08
• Beilstein: 103853
• Numer WE: 206-019-2
• Numer MDL: MFCD00005183
• Kod UNSPSC: 12352005
• eCl@ss: 39161001
• Identyfikator substancji w PubChem: 24896072
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• ciśnienie pary: <1 mmHg ( 20 °C)
• Próba: ≥99% (titration)
• Postać: crystalline powder
• warunki przechowywania: dry at room temperature
• metody: electrophoretic mobility shift assay: suitable; molecular cloning: suitable; protein purification: suitable
• kolor: white
• pH: 6.2-7.8 (25 °C)
• pKa (25°C): 6.95
• tw: 256 °C (lit.)
• mp: 88-91 °C (lit.)
• rozpuszczalność: water: 500 mg/mL, clear, colorless to very faintly yellow
• gęstość: 1.004 g/cm³ at 20—25 °C; 1.006 g/cm³ at 20—25 °C
• przydatność: suitable for molecular biology
• Zastosowanie: diagnostic assay manufacturing; genomic analysis; life science and biopharma; microbiology
• obecność zanieczyszczeń: DNase, RNase and protease, none detected
• temp. przechowywania: room temp
• ciąg SMILES: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• Klucz InChI: RAXXELZNTBOGNW-UHFFFAOYSA-N

Opis

Opis ogólny

Imidazole, also known as Glyoxalin, is an aromatic heterocyclic diazole and a key biological buffer with a pH range of 6.2 to 7.8 at 25°C. It has a wide range of applications in molecular biology, biochemistry, and proteomics. In proteomics, imidazole is essential for eluting histidine-tagged proteins from IMAC columns. It is a strong chelator of divalent cations, such as nickel and zinc, which disrupts the interaction between the His-tag and the metal ions. This allows for the efficient elution of purified proteins.

Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.

Zastosowanie

Imidazole has been used

• in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
• in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
• as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
• as a binding buffer, wash buffer, and elution buffer in a study involving protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture

Excellent for buffers in the range of pH 6.2-7.8

Działania biochem./fizjol.

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

Cechy i korzyści

• Suitable for Molecular Biology and Biochemical Research
• Can be used as a Buffer component, for Protein separation
• Free from DNase, NICKase, RNase, and Protease
• Effective Buffering from pH 6.2 to 7.8 (25 °C) with a pKa of 6.9 (25 °C)

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302,H314,H360D
• Zwroty wskazujące środki ostrożności: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): 293.0 °F - closed cup
• Temperatura zapłonu (°C): 145 °C - closed cup
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges