Prior to determining the activity of this enzyme, Sigma reconstitutes it in 250 mM glycylglycine buffer, pH 7.4.
Przejdź do
H8629
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
lyophilized powder
Synonim(y):
HK/G6PD
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Do Państwa/SKU | Dostępność | Cena netto |
|---|
Informacje o tej pozycji
Informacje o cenach i dostępności nie są obecnie dostępne.
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócform
lyophilized powder
Quality Segment
glucose-6-phosphate dehydrogenase activity
≥75 units/mg protein (biuret)
hexokinase activity
≥65 units/mg protein
purified by
crystallization
storage temp.
−20°C
General description
Research area: Cell signaling
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49
Application
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.
Biochem/physiol Actions
Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.
Packaging
Package sizes are based on hexokinase units.
Other Notes
Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D
Mixture of hexokinase and glucose-6-phosphate dehydrogenase
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description lyophilized powder | description Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian) | description Type VII, ammonium sulfate suspension, ≥200 units/mg protein | description lyophilized powder, ≥50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| purified by crystallization | purified by crystallization | purified by - | purified by - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| hexokinase activity ≥65 units/mg protein | hexokinase activity - | hexokinase activity - | hexokinase activity - |
| glucose-6-phosphate dehydrogenase activity ≥75 units/mg protein (biuret) | glucose-6-phosphate dehydrogenase activity - | glucose-6-phosphate dehydrogenase activity - | glucose-6-phosphate dehydrogenase activity - |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
How should Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be reconstituted?
1 answer-
Helpful?
-
-
What units are the package sizes of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, based upon?
1 answer-
This product is packaged on the basis of hexokinase units.
Helpful?
-
-
What is the stability of a stock solution of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
1 answer-
Stock solutions prepared in water or 10 mM potassium phosphate buffer pH 8.0 lose approximately 1% activity per month when stored at 4°C.
Helpful?
-
-
Does Sigma offer any other product which can be used in place of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
1 answer-
Yes. We offer Product No. G3293 (Glucose Assay Reagent), which is a component of the GAHK20 (Glucose Assay Kit).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Can Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be used for the determination of glucose?
1 answer-
A method for the determination of glucose using hexokinase and glucose 6-phosphate dehydrogenase is described in "Methods of Enzymatic Analysis" 3rd ed., Vol. VI, p. 163.
Helpful?
-