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F6765

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

Synonim(y):

Fetal Calf Serum, FBS, FCS, sera, serum

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100 ML
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500 ML
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Wybierz wielkość

Zmień widok
100 ML
Prześlij zapytanie
500 ML
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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.71
pochodzenie biologiczne:
bovine fetus
pochodzenie:
USA origin
sterylność:
sterile-filtered
Warunki transportu:
dry ice


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

pochodzenie biologiczne

bovine fetus

Poziom jakości

sterylność

sterile-filtered

linia produktu

FBS Select

skład

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

pochodzenie

USA origin

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Zastosowanie

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Charcoal stripped FBS is often used instead of FCS to minimize the level of androgen and other hormones provided in 10% serum supplemented media.

Działania biochem./fizjol.

Charcoal Stripped Fetal Bovine Serum (FBS) is available for researchers requiring decreased levels of various hormones in their studies. Dextran treated Charcoal is used to selectively remove hormones without nonspecific loss of other serum components. The effectiveness of hormone removal is assessed on the basis of reductions in the levels of T3, T4, and Progesterone pre and post treatment.

This product is designed for researchers involved in studying processes influenced by steroid hormones such as estrogen stimulation and the obesity process and other areas that benefit from reduced hormone levels such as certain types of viral infections.

Komentarz do analizy

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Inne uwagi

The product is not available in Australia. Please view our protocol for Charcoal-stripping FBS to deplete hormones here.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


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Certyfikaty analizy (CoA)

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Produkty

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Zapoznaj się z podstawami stosowania płodowej surowicy bydlęcej (lub cielęcej) do hodowli komórek oraz z tym, jak pozyskiwanie i przepisy wpływają na cenę surowic do testów komórkowych.

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Protokoły

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Chroń swoją inwestycję dzięki najlepszym praktykom przechowywania i rozmrażania FBS i innych surowic. Próbki FBS do walidacji w protokole hodowli komórkowej.

Powiązane treści

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Questions

1–2 of 2 Questions  
  1. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer
    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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