F4229

Monoclonal Anti-Fas (CD95/Apo-1)−FITC antibody produced in mouse

clone DX2, purified immunoglobulin, buffered aqueous solution

• Synonim(y): Monoclonal Anti-Fas, Anti-Apo-1, Anti-CD95
• Kod UNSPSC: 12352203

Właściwości

• pochodzenie biologiczne: mouse
• białko sprzężone: FITC conjugate
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: DX2, monoclonal
• Postać: buffered aqueous solution
• reaktywność gatunkowa: human
• metody: flow cytometry: 1:50 using Cultured human Burkitt′s lymphoma Raji cells
• izotyp: IgG1
• numer dostępu UniProt: P25445
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• informacje o genach: human ... FAS(355)

Opis

Opis ogólny

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

Specyficzność

Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.

Immunogen

murine L cells transfected with a human Fas/CD95 cDNA.

Zastosowanie

Monoclonal Anti-Fas (CD95/Apo-1)-FITC antibody is suitable for flow cytometry at a dilution of 1:50 using cultured human Burkitt′s lymphoma Raji cells.

Działania biochem./fizjol.

APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

This page may contain text that has been machine translated.