The activity was found by taking the units/ml of enzyme and dividing it by the mg protein/ml of enzyme. The activity was found in an spectrophometric rate determination assay. The result found that one unit of enzyme will oxidize 1.0 micro mole of formaldehyde to formic acid per minute at pH 7.5 at 37°C.
F1879
Formaldehyde Dehydrogenase from Pseudomonas sp.
lyophilized powder, 1.0-6.0 units/mg solid
Synonim(y):
FDH, Formaldehyde:NAD+ oxidoreductase
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Informacje o tej pozycji
Specific activity:
1.0-6.0 units/mg solid
Przejdź do
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócform
lyophilized powder
specific activity
1.0-6.0 units/mg solid
storage temp.
−20°C
General description
Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family.[1] It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa.[2] The FDH sequences of P. aeruginosa and P. putida are conserved and show homology.[1] FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.[3]
Application
Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:
- For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1)[4] and in formaldehyde dehydrogenase (FDH) assay.[5]
- As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.[6]
- In coupled pectin methyl esterase (PME) enzyme assay.[6]
Biochem/physiol Actions
Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.[1]Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide.[2] FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione.[7] They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).[8]
Physical form
Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine
Other Notes
One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
In Vitro Enzyme Assays for JmjC-Domain-Containing Lysine Histone Demethylases (JmjC-KDMs)
Tarhonskaya H, et al.
Current protocols in pharmacology, 80(1), 3-15 (2018)
Redundancy of enzymes for formaldehyde detoxification in Pseudomonas putida
Roca A, et al.
Journal of Bacteriology, 191(10), 3367-3374 (2009)
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+
Liao Y, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 69(Pt 9), 967-972 (2013)
Ivan V Rosado et al.
Nature structural & molecular biology, 18(12), 1432-1434 (2011-11-15)
Metabolism is predicted to generate formaldehyde, a toxic, simple, reactive aldehyde that can damage DNA. Here we report a synthetic lethal interaction in avian cells between ADH5, encoding the main formaldehyde-detoxifying enzyme, and the Fanconi anemia (FA) DNA-repair pathway. These
-
この酵素の活性値を測定した方法について教えてください。
1 answer-
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej