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Merck

E7033

pFLAG-CMV-2 Expression Vector

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

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UNSPSC Code:
12352200
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Nazwa produktu

pFLAG-CMV-2 Expression Vector, shuttle vector for intracellular transient expression of N-terminal Met-FLAG

tag

FLAG® tagged

grade

Molecular Biology

form

buffered aqueous solution

quality

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

peptide tag location

N-terminal

shipped in

dry ice

storage temp.

−20°C

Biochem/physiol Actions

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

General description

The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Other Notes

  • pFLAG-CMV-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
  • pFLAG-CMV-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
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Klasa składowania

10 - Combustible liquids


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Li Yang et al.
Cancer research, 67(12), 5587-5593 (2007-06-19)
Evidence indicates that the induction of cyclooxygenase-2 (COX-2) and high prostaglandin E2 (PGE2) levels contribute to the pathogenesis of non-small-cell lung cancer (NSCLC). In addition to overproduction by COX-2, PGE2 concentrations also depend upon the levels of the PGE2 catabolic
Jing Ouyang et al.
Blood, 117(16), 4315-4322 (2011-02-09)
Posttransplant lymphoproliferative disorders (PTLDs) are potentially fatal, EBV-driven B-cell malignancies that develop in immunocompromised solid organ or hematopoietic stem cell recipients. In PTLD, the expression of EBV proteins, including latent membrane protein 1 (LMP1) and LMP2A, viral immune evasion strategies
Michiko Tanaka et al.
Virology journal, 5, 125-125 (2008-10-23)
The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae. Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the involvement of HSV-1 UL7 in viral replication has been
Li Zhu et al.
Reproduction (Cambridge, England), 139(4), 717-731 (2010-02-05)
Retinoic acid receptor alpha (RARA) is critical for spermatogenesis, as shown by a sterility phenotype observed in Rara knockout mice. RARA is important in both Sertoli and germ cells of the testis. Here, we demonstrate that a disulfide isomerase glucose-regulated
William T Arthur et al.
The Journal of cell biology, 167(1), 111-122 (2004-10-14)
The Ras-related GTPase Rap1 stimulates integrin-mediated adhesion and spreading in various mammalian cell types. Here, we demonstrate that Rap1 regulates cell spreading by localizing guanine nucleotide exchange factors (GEFs) that act via the Rho family GTPase Rac1. Rap1a activates Rac1

Powiązane treści

pFLAG-CMV-1 and pFLAG-CMV-2 Vector Maps

Instructions

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