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DNASE10

On-Column DNase I Digestion Set

sufficient for 10 preparations

Synonim(y):

Deoxyribonuclease I Set

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1 SET

248,00 zł

248,00 zł

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Informacje o tej pozycji

NACRES:
NA.52
UNSPSC Code:
12352204

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Pozwól nam pomóc

grade

Molecular Biology

form

liquid

usage

sufficient for 10 preparations

technique(s)

RNA purification: suitable

storage temp.

−20°C

Quality Level

200

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Ta pozycja
D7291DNASE70AMPD1
On-Column DNase I Digestion Set sufficient for 10 preparations

Sigma-Aldrich

DNASE10

On-Column DNase I Digestion Set

Deoxyribonuclease I RNase-free solution from bovine pancreas

Sigma-Aldrich

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

On-Column DNase I Digestion Set sufficient for 70 preparations

Sigma-Aldrich

DNASE70

On-Column DNase I Digestion Set

DNase I Amplification Grade

Sigma-Aldrich

AMPD1

DNase I

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

technique(s)

RNA purification: suitable

technique(s)

DNA extraction: suitable

technique(s)

RNA purification: suitable

technique(s)

RT-PCR: suitable

form

liquid

form

buffered aqueous glycerol solution

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 10 preparations

usage

-

usage

sufficient for 70 preparations

usage

-

General description

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit and the Spectrum Plant Total RNA Kit. It can also be used with many other commercially available RNA purification kits.
The vast majority of DNA is eliminated from total RNA purifications by the procedures provided in the standard protocols of the Sigma Kits named below. However, for very sensitive applications, such as quantitative RT-PCR, even trace amounts of DNA can result in false positive detection of cDNA-derived amplicons.

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

The term ′On-Column′ indicates that digestion is accomplished in-process for true convenience. Each of Sigma′s total RNA purification kits utilize a binding column for purification. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow. To review the complete protocol, see the Technical Bulletin for any of the Kits named above.

Application

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.[1]

Biochem/physiol Actions

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.

Features and Benefits

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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Tylko elementy zestawu

Numer produktu
Opis
  • DNase Digestion Buffer
  • Deoxyribonuclease I

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000469650
0000469650
SLCR5423
SLCR1901
SLCN1907

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Carmen Michán et al.
FEMS yeast research, 9(7), 1078-1090 (2009-08-26)
We report the absolute transcription profiles of 24 genes coding for putative thioredoxin (Trx)- and glutathione (GSH)-dependent redox system components, accompanying the Candida albicans batch culture growth, under either yeast or hyphal conditions. All mRNAs investigated (plus the housekeeping ACT1)
Filipa Monteiro et al.
PloS one, 8(9), e72998-e72998 (2013-09-12)
The pivotal role of cultivated grapevine (Vitis vinifera L.) in many countries economy is compromised by its high susceptibility to Plasmopara viticola, the causal agent of downy mildew disease. Recent research has identified a set of genes related to resistance
Michal Bukowski et al.
Nature communications, 4, 2012-2012 (2013-06-19)
Toxin-antitoxin systems were shown to be involved in plasmid maintenance when they were initially discovered, but other roles have been demonstrated since. Here we identify and characterize a novel toxin-antitoxin system (pemIKSa) located on Staphylococcus aureus plasmid pCH91. The toxin

Protokoły

Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits

Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.

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Zestaw Spectrum™ Plant Total RNA Kit

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Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

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