ATHOS

93122325, Porcine thyroid, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: ATHOS, 93122325
• pochodzenie biologiczne: Porcine thyroid
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Thyroglobulin, plasminogen activator (u-PA)
• receptory: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Porcine thyroid epithelial

Opis linii komórkowej

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

Zastosowanie

Mechanism of thyroid follicle reconstruction

pożywka hodowlana

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Inne uwagi

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