M3 Clone M-3

93120827, mouse skin, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: M3 Clone M-3, 93120827
• pochodzenie biologiczne: mouse skin
• tryb wzrostu: Adherent
• kariotyp: Modal no. 83; hypertetraploid
• morfologia: Epithelial
• produkty: Melanin
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse Cloudman S 91 melanoma

Opis linii komórkowej

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Zastosowanie

Tumourigenicity studies

pożywka hodowlana

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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