A9(Hamprecht)

8062526, mouse connective tissue, Fibroblast

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: A9(Hamprecht), 08062526
• pochodzenie biologiczne: mouse connective tissue
• tryb wzrostu: Adherent
• kariotyp: Modal No of chromosomes is 54
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse Fibroblast (L cells)

Opis linii komórkowej

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

Zastosowanie

Used in the generation of cell hybrids.

pożywka hodowlana

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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