KARPAS-25

6072601, human lung (effusion), Round to polygonal cells growing singly in suspension

• Synonim(y): KARPAS 25, KARPAS, 25, KARPAS25
• Kod UNSPSC: 41106514

Właściwości

• product name: KARPAS-25, 06072601
• pochodzenie biologiczne: human lung (effusion)
• tryb wzrostu: Suspension
• kariotyp: Hyperdiploid with complex aberrant chromosomes
• morfologia: Round to polygonal cells growing singly in suspension
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice

Opis

Pochodzenie linii komórkowej

Human acute plasma cell leukaemia

Opis linii komórkowej

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

pożywka hodowlana

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Rutyna subkultury

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.